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q-fDOM

Improving the quality of fluoresence based dissolved organic matter observations in coastal waters

Funded by AQUARIUS Transnational Access Call 2

Coastal seas are the hotspots in global marine carbon fluxes. They receive considerable amounts of organic carbon originating from terrestrial and freshwater systems through rivers and estuaries.

Region of Operation: North Sea and the Baltic Sea

Research Infrastructure Used: MESO & CAL, Alg@line, NorSOOP

“The q-fDOM project has gathered a fantastic group of experts and users of fluorescence-based measurements of CDOM and will work towards harmonization of the commercially available fDOM fluorometers.”

Anna Willstrand Wranne, Voice of the Ocean Foundation

PARTNERS

Positions for collection of water samples for the CDOM mesocosm experiments as well as pictures from the SYKE mesocosm facility.

MAIN OBJECTIVES

Coastal seas are the hotspots in global marine carbon fluxes (Dai et al 2022). They receive considerable amounts of organic carbon originating from terrestrial and freshwater systems through rivers and estuaries. Compared to the open ocean, coastal seas often have high biological activity, both in producing new organic matter through photosynthesis and in mineralising the existing organic matter pools. In coastal waters the increase of dissolved organic matter (DOM) causes brownification, a change in water colour and increase of light attenuation, affecting light availability for primary productivity. DOM also contains nutrients that may boost primary production or bacterial processes, and the labile carbon in DOM will induce mineralisation processes. Thus, DOM fluxes affect the aquatic metabolism and determine if the system is a source or sink of oxygen and carbon. Terrestrial carbon is also a key determinant of carbon budgets in many coastal systems and riverine inputs have affects the carbonate system (alkalinity, DIC, pH, pCO2). A high-quality pan-European monitoring network to study coastal carbon dynamics and transport of carbon from terrestrial systems to coastal seas and further to open ocean has been proposed by ICOS, Euro ARGO, and JERICO communities (Felgate et al 2020, Frigstad et al 2024).Optimally, such a monitoring system includes measurements of various carbon fractions, related carbonate system variables (TA, pH) and optical proxies for dissolved organic matter  – coloured dissolved organic matter (CDOM), and the fraction of CDOM that fluoresces (fDOM) (Felgate et al 2020). The user group, including six partners doing Carbon-related research and monitoring in the Baltic Sea and North Sea, have the goal to harmonize and standardize fDOM measurements in order for harmonisation of methods and adoption of the best practices that is required to yield consistent data across the studied systems.

Main objectives:

  1. Share knowledge on the use, calibration and data-correction procedures for fDOM sensors among partnerships, leading towards development of pan-European best practices in fDOM sensor calibration and validation.
    During a 1-week workshop, the user group and facility owners share their experience in fDOM sensor use and calibration, hand-on comparison of some key methods is done and the most suitable method is selected for calibrating sensors in this project.
  2. Comprehensive experimental determination of fDOM temperature dependency for various sensors across the different water types and the determination of optical variations among sensors.
    Partnership provides a wide range of coastal waters to be tested, using the available range of commercial sensors. Results will highlight if the obtained correction functions are “universal” or if there are differences between sensors (with different optics) and between water types (with different DOM quality). Additional information is gained on how the optical match-up between DOM spectral excitation-emissions properties and sensor spectral properties, and fluorescence re-absorption may affect the readings.

  3. Test how consistent data various sensors provide across temperature and fDOM gradients, guiding developments towards harmonized sensor networks.

    Long-term in situ tests, deploying several sensors simultaneously in Ferrybox systems, will reveal if consistent data is obtained after joint calibration of sensors (1) and after applying best available experimentally derived corrections for temperature and optics (2). Recommendations will be reported for build-up of Carbon monitoring networks, for fDOM sensor calibration, corrections and validation, and for sensor selection (see Section 7).

MORE INFORMATION

AQUARIUS TA Project Name:

Improving the quality of fluoresence based dissolved organic matter observations in coastal waters

Project acronym:

q-fDOM

TA Project Unique ID:

61

Partners involved:

• Voice of the Ocean Foundation
• OceanObs
• Swedish Meteorological and Hydrological Institute (SMHI)
• Leibniz-Institut für Ostseeforschung Warnemünde (IOW)
• Helmholz Zentrum Hereon
• Technical University of Denmark (DTU)

Start date 

1 May 2026

End date 

1 June 2027

Mission Lighthouse Region

Baltic & North Sea

Work/research geographical
area

North Sea and the Baltic Sea

Names of RI facilities used

• MESO & CAL
• Alg@line
• NorSOOP

Scientific discipline(s)

Earth Sciences & Environment: Marine science/Oceanography

Principal Investigator
(PI) Affiliation

Anna Willstrand Wranne